CLARITY forum

Discussion List

• Two alternatives to the nitrogen purge for the hydrogel

  886 views 7 comments 6 points Most recent by BJ2014 March 2015Hydrogel Embedding
• Is A4P0 w/ photoinitiator stable in 4degC fridge?

  93 views 2 comments 0 points Most recent by BJ2014 March 2015Hydrogel Embedding
• FocusClear, amount?

  161 views 5 comments 0 points Most recent by Adam February 2015CLARITY Materials
• Why a Hydrogel solution?

  184 views 2 comments 0 points Most recent by marine_dubois01 February 2015Hydrogel Embedding
• CLARITY + iDISCO

  325 views 4 comments 0 points Most recent by lorenzoo February 2015Sample Imaging
• SDS and acrylamide concentrations

  180 views 2 comments 0 points Most recent by pavlovaip February 2015Electrophoretic Tissue Clearing (ETC)
• 3D printable chamber

  88 views 1 comment 0 points Most recent by WFD February 2015Sample Imaging
• Gel polymerization does not work

  1.3K views 15 comments 0 points Most recent by Adam January 2015Hydrogel Embedding
• Autofluorescence

  4.7K views 41 comments 0 points Most recent by Jan January 2015Sample Imaging
• Post pictures of cleared tissue

  2.1K views 17 comments 0 points Most recent by hydrazine January 2015Electrophoretic Tissue Clearing (ETC)
• Make sample slice before passive clearing?

  59 views 3 comments 0 points Most recent by Fabio January 2015General
• PBST solution - .1% triton X v/v , w/w , w/v?

  124 views 2 comments 0 points Most recent by hydrazine January 2015General
• Sample incredibly squishy after passive clearing.

  30 views 1 comment 0 points Most recent by Adam January 2015General
• Sample swelling

  222 views 4 comments 0 points Most recent by Fabio January 2015General
• Chicago, IL

  69 views 1 comment 0 points Most recent by mjisarobot December 2014General
• Raw data of the rat entorhinal cortex and hippocampus?

  347 views 0 comments 0 points Started by Martin_Pyka December 2014 Data Analysis
• 3DISCO imaging setup / imaging chamber

  682 views 1 comment 0 points Most recent by nicolasrenier December 2014Sample Imaging
• Validated antibodies

  197 views 0 comments 0 points Started by JLM December 2014 Sample Staining
• 0.5% acrylamide concentration, no polymerization?

  336 views 4 comments 0 points Most recent by DUJ December 2014Hydrogel Embedding
• Has anyone prepared the hydrogel soln. with 4% PFA?

  74 views 1 comment 0 points Most recent by Adam December 2014Hydrogel Embedding
• Refractive index matching solution from Cargille Labs

  158 views 0 comments 0 points Started by Adam December 2014 Sample Imaging
• Are proteins denatured during the clearing proccess?

  49 views 1 comment 0 points Most recent by Adam December 2014General
• What causes the tissue to swell in the clearing solution?

  28 views 0 comments 0 points Started by hydrazine December 2014 General
• Temperatures above 37 deg C?

  100 views 2 comments 0 points Most recent by rchurt November 2014Hydrogel Embedding
• Perfusion in rats

  187 views 3 comments 0 points Most recent by JAGS November 2014Hydrogel Embedding
• 0.3 mm Pt wire diameter

  58 views 2 comments 0 points Most recent by Jan November 2014CLARITY Materials
• Tisssue swelling and flaking

  162 views 4 comments 0 points Most recent by balsorjl October 2014General
• Immunohistochemistry in the process

  249 views 2 comments 0 points Most recent by JAGS October 2014Sample Imaging
• Refractive index mismatch in confocal

  166 views 0 comments 0 points Started by LinusMG October 2014 Sample Imaging
• Neurolucida and Fluoview FV10i - (or general imaging help)

  109 views 1 comment 0 points Most recent by LinusMG October 2014Sample Imaging