Refractive index mismatch in confocal
LinusMG
Posts: 20
Hello everyone,
I was wondering whether there are any easy solutions for the refractive index mismatch when using general air or water-immersion objectives in confocal set-ups.
In SPIM set-ups this is easy to correct, at the end using one lens the light can be re-focused.
By now in confocal this issue seems to be solved using multi-immersion lenses or refractive-index matched objectives.
Does anyone know any generic correction collars that could be attached to any objective?
Or have anyone came up with a nice solution?
I was wondering whether there are any easy solutions for the refractive index mismatch when using general air or water-immersion objectives in confocal set-ups.
In SPIM set-ups this is easy to correct, at the end using one lens the light can be re-focused.
By now in confocal this issue seems to be solved using multi-immersion lenses or refractive-index matched objectives.
Does anyone know any generic correction collars that could be attached to any objective?
Or have anyone came up with a nice solution?