CLARITY forum

Discussion List

• Electro-immunofluorescence staining for better Ab staining of thick tissues--thoughts?

  164 views 1 comment 0 points Most recent by jdscott March 2014Sample Staining
• CLARITY of Skeletal Muscle

  483 views 4 comments 1 point Most recent by neuro100 March 2014General
• CLARITY users London (UK) Informal Meeting Early 2014 (January/February)

  383 views 5 comments 1 point Most recent by TomPhillips March 2014General
• Electrode material/diameter

  39 views 0 comments 0 points Started by DUJ March 2014 CLARITY Materials
• Deep antibody penetration in CLARITY-processed tissue, using proteinase K

  704 views 7 comments 0 points Most recent by KeithSiew March 2014Sample Staining
• Microscopy Setups - What works best?

  1.4K views 19 comments 0 points Most recent by Matasha_D March 2014Sample Imaging
• Gel collapsed =(

  98 views 1 comment 0 points Most recent by Varf February 2014Hydrogel Embedding
• Passive clearing of mouse brain tissue !

  326 views 1 comment 0 points Most recent by Kristin_Engberg February 2014Electrophoretic Tissue Clearing (ETC)
• Sample burned while ETC ?

  211 views 4 comments 0 points Most recent by joelrosiene January 2014Electrophoretic Tissue Clearing (ETC)
• 1 M sodium borate buffer solution

  37.8K views 6 comments 0 points Most recent by Shashank20 January 2014Sample Staining
• Saturation of tissue before staining and buffer of incubation

  137 views 1 comment 0 points Most recent by Shashank20 January 2014Sample Staining
• ETC Chambers - Purchasing or Borrowing

  40 views 0 comments 0 points Started by hschand January 2014 Electrophoretic Tissue Clearing (ETC)
• Imaging samples whose proteins could be denatured?

  66 views 2 comments 0 points Most recent by patricia January 2014Sample Imaging
• Incubation time in SDS clearing solution

  274 views 4 comments 0 points Most recent by yetiti January 2014Electrophoretic Tissue Clearing (ETC)
• White precipitate on tissue during electrophoresis

  56 views 2 comments 0 points Most recent by DominicJG January 2014Electrophoretic Tissue Clearing (ETC)
• FocusClear in the UK

  170 views 7 comments 0 points Most recent by KeithSiew January 2014CLARITY Materials
• How long can I leave sample before polymerization?

  167 views 3 comments 0 points Most recent by LauraLynch January 2014Hydrogel Embedding
• Immunostaining of CLARITY-processed post-mortem human brain tissue

  162 views 0 comments 0 points Started by Irene December 2013 Sample Staining
• Unelectrophoresed, but clear...

  990 views 14 comments 4 points Most recent by Fabio December 2013General
• Scanning Time

  145 views 4 comments 0 points Most recent by DominicJG December 2013Sample Imaging
• Loss of GFP Signal

  3.2K views 21 comments 0 points Most recent by KeithSiew December 2013Hydrogel Embedding
• CLARITY on Mouse Spleen

  126 views 1 comment 0 points Most recent by Elliott November 2013General
• SDS Backorder, other options?

  170 views 3 comments 0 points Most recent by Elliott November 2013CLARITY Materials
• Insect tissue

  35 views 0 comments 0 points Started by matthewterry November 2013 General
• Hydrogel polymerized in the fridge while thawing...

  123 views 0 comments 0 points Started by jroc093 November 2013 Hydrogel Embedding
• Chamber Current and Voltage as They Relate to Sample Clearing and Degradation

  1.9K views 21 comments 0 points Most recent by joelrosiene November 2013Electrophoretic Tissue Clearing (ETC)
• has anyone tried CLARITY on Xenopus embryos?

  53 views 0 comments 0 points Started by maura November 2013 General
• We finally got it to work with viral injection.

  330 views 0 comments 0 points Started by LauraLynch November 2013 Sample Imaging
• rat brain

  233 views 2 comments 0 points Most recent by ferdi October 2013General
• CLARITY of Embryos

  1.1K views 14 comments 1 point Most recent by nicolasrenier October 2013General