We have obtained our first partially cleared samples and now we wanted to try immunofluorescence on those samples. We have difficulties dissolving and preparing the 1 M sodium borate buffer solution, pH 8.5. Could somebody post a recipe for preparing it? Thank you in advance.
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After staining and washing, do you go straight to focusclear? Does the brain need to be washed in focusclear for a few days and then fresh focusclear used for imaging?