I want to stain my cleared mouse brain, but I look the protocol described in the publication, there is no saturation of the aspecific site with serum from the 2nd antibody specie. Has anybody tried to stain samples including this step before the incubation with the first antibody? has anybody tried to incubate the antibodies in other solution that the sodium borate buffer?
Cheers
Christelle
0 ·
Comments