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Samples should always be washed and stored in PBS after the initial imaging since most, if not all RI match solutions, are not storage solutions. If it is for long-term storage it needs to be in PBS … (View Post)

Here is our chamber. It is made w/ .2mm We make as much as possible out of polycarb and/or acrylic so that we can solvent weld it. The wire ports were sealed w/aquarium silicone. We also used it on t… (View Post)

Hi everyone, I just wanted to send a reminder that the Deisseroth lab offers training workshops on the CLARITY technique if you'd like a chance to learn and troubleshoot in-person. More information c… (View Post)

I have not tried the CLARITY method yet, but some have reported difficulties with polymerisation. I noticed when preparing polyacrylamide gels for electrophoresis that plastics, e.g. vinyl tubing giv… (View Post)

Hi, maybe you can try the method of C.A.P.T.U.R.E. (CLARITY-based Activity and Projection Tracking upon Recombination, http://capture-clarity.org/) published in CELL this year. It’s easier than ETC a… (View Post)

From my personal experience, expansion may be caused by osmotic difference by the clearing solution used. Even without electric current, tissue will be expanded in the clearing solution. The best way… (View Post)

I have experienced this before and my tissue block turned a bit opaque and yellow after 37 degrees glycerol immersion for 2 days. So I tested out the optimal time for the glycerol incubation in order… (View Post)

I'm using a Cole-Parmer EW-77910-30 peristaltic pump (~$900 new) set at about 150 ml/min to pump buffer from a 2L bottle in a 37C water bath into the chamber. The chamber then drains back into the bo… (View Post)

The jellyness is normal. The clarified samples are weak and must be handled very carefully. Could the flaking be due to any false move with the sample? I would wait until the brain is completely clar… (View Post)

We will be using CLARITY to image mouse skeletal muscle, but we are wondering if anyone has tried CLARITY on any muscle samples? We are aware that the cartilage and tendons in the muscle might prove … (View Post)