-
Re: Loss of GFP Signal
Thanks Laura, Richard - I'm currently the process of clearing a brain with a high amount of native fluorescence. I'll mount sections of it in FauxcusClear, ScaleU2, and ScaleA2 to try to ge…1 -
Re: brass in contact with buffer
At one point I had a bronze electrode nut open to the system. Very bad corrosion and blue residue was the result. Now I waterproof everything with a sealant that isn't platinum.1 -
Re: FocusClear
Just reporting here that I have had some success using a saturated solution of diatrizoic acid in DMSO, with NaOH at a 50% Molar ratio to the diatrizoic acid, and a 0.1% Tween20. The only issue I hav…1 -
Re: Water circulator
I use a Zoomed TCN30, which circulates at 5 liters per minute. I found that the circulating speed was a little fast, so I included a wall within my chamber which breaks up the turbulence and decrease…3 -
Re: Chamber Current and Voltage as They Relate to Sample Clearing and Degradation
The brains do move, but not by much, they merely bobble around within the room that I give them. I have a mesh cap on each end of the clear section, before the electrode, to prevent the brain from co…1
