@LinusMG My first instinct is to say that flow rate is primarily relevant to the management of the temperature of the chamber. If you can get away with a lower flow rate and still hold the temperatur… (View Post)
I cannot imagine that adipose tissue would undergo clarity very well - there may not be enough non-lipid structure to remain after ETC. However, I am not speaking from experience. (View Post)
I'm pretty sure that tough gel is indicative of good polymerization. Personally I no longer attempt to cut each brain out of the gel. Now I just cup the tips of my fingers around the brain, and with … (View Post)
The authors of the SeeDB paper also stated that they had to get a manufacturer to design them a customized objective in order to image using their 80-90% thiodiethanol, because it has a really high R… (View Post)