I've found that, once most of the surrounding gel is removed, gently rolling the tissue on a paper towel or Kimwipe helps get rid of the rest. (View Post)
Passive clearing greatly reduces autofluorescence in our brain tissue. Also, don't run ETC at high voltage, high temperature, nor for more than a day or so. (View Post)
We use a dessicator from McMaster-Carr, part number 2204K5: http://www.mcmaster.com/#catalog/119/671/=oym46z Inside we have a piece of styrofoam from the package that our Corning 50 ml tubes comes in… (View Post)
About buffer color change: I've noticed that ours turns yellowish after a few days of clearing. I change the buffer every two days (when the pH drops below 8.2). From the looks of your photos, I thin… (View Post)