Dont`t put samples that are in clearing solution in temperatures below RT. If you do this the SDS will precipitate. If you want to store the samples PBST is good, for long-term 4° PBST + diluted azid… (View Post)
Did you wash long enough in PBST? Could be residues from the SDS that precipitate at temp lower 15 °C also your buffer is really cloudy... shouldt be (View Post)
You will see autofluorescence under a stereomicroscope, but once I go to the confocal I see my signal. My mice have the GFP coupled to histone H2B. So you have a strong nuclear signal. (View Post)
Leaving the bath open doesnt cahnge color (for me). You have evaporation and loss of buffer volume, which has an effect on ion concentration and SDS micelles. The problems 1 & 2 & 3, I would … (View Post)
I ordered from german wako. http://www.wako-chemicals.de/Laborchemikalien/130764,1031,111427,-1.aspx the cat nr. of the VA-044 is 017-19362 just paste it in the link below. http://www.e-reagent.com/e… (View Post)