Adam

Glycerol works much better for thinner samples. In my experience, it's fine up to about 3-4mm, but can't be used for whole or half brains. (View Post)

1:100 is usually where I start with most antibodies, and this worked with GFAP for me (in mouse tissue). (View Post)

iDISCO is a staining/clearance technique, do you mean using a lightsheet/SPIM system for imaging? (View Post)

I vaguely assumed that SDS had less of an effect upon PFA fixed tissue, but I don't really know. I'd be interested if any one else has any input. (View Post)