CLARITY and FISH

Claire_Zhang · June 2013

Technically, could the tissue went through CLARITY be used for FISH (Fluorescence in situ hybridization)? As far as I know the size of a regular DNA probe is smaller than that of a common antibody. Would the hydrogel fit for the other conditions required for FISH? What are the precautions would be? Thank you!

Adam · February 2015

To resurrect this, has anyone tried FISH with CLARITY, other than the single figure panel in the original paper?

festeves · May 2015

Necro once more. Any word on this ?

Jarbuckle · November 2015

If you are working with tissue sections, you will find an improvement in imaging performance by simply using a few drops of EasyIndex on the section, post staining. This will increase your signal to noise ratio also. EasyIndex rapidly clears sections less than 20um. A clearing protocol is not necessary for this range of tissue thickness.

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CLARITY and FISH

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