CLARITY and FISH

Claire_Zhang

Technically, could the tissue went through CLARITY be used for FISH (Fluorescence in situ hybridization)? As far as I know the size of a regular DNA probe is smaller than that of a common antibody. Would the hydrogel fit for the other conditions required for FISH? What are the precautions would be? Thank you!

Adam

To resurrect this, has anyone tried FISH with CLARITY, other than the single figure panel in the original paper?

festeves

Necro once more. Any word on this ?

Jarbuckle

If you are working with tissue sections, you will find an improvement in imaging performance by simply using a few drops of EasyIndex on the section, post staining. This will increase your signal to noise ratio also. EasyIndex rapidly clears sections less than 20um. A clearing protocol is not necessary for this range of tissue thickness.