Optical Projection Tomography

KatherineHolzem

Does anyone currently have (or is in the process of constructing) an OPT system? My PI told me about this imaging method, and now we are hoping to build one for our department. So if anybody has any pointers, that would be great. Seems like potentially one of the better methods for large-sample imaging with clear tissue, but has mostly been used for developmental/embryonic imaging since it came out >10 years ago.

If you don't know about OPT, there are a few great papers out there. I have cited a couple below.

The original OPT publication: Sharpe et al. Science. 2002.
A recent publication about constructing your own OPT imaging system: Wong et al. PLOSone. 2013.

Shashank20

Hi Katherine we are quite often using OPT for imaging mouse/rat CNS and mouse/human pancreas. I am pasting recently accepted manuscript on CNS-OPT and other one with human pancreas imaged using OPT.
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076330
http://onlinelibrary.wiley.com/doi/10.1111/ajt.12138/abstract

JosephineSnow

A video paper on OPT for use in imaging beta cell mass in the Pancreas: http://www.jove.com/video/50238/near-infrared-optical-projection-tomography-for-assessments-cell-mass