I noticed in the brains I've cleared there has been a white precipitate forming when placed in Focus Clear, and when viewed under the microscope there are very noticeable holes in the tissue. We think it may be the result of the way we made the clearing solution. When making the solution, we just added the boric acid and sds, followed by the dH2O. A white precipitate formed, and it took more than 200 pellets of NaOH (for 10L of clearing solution) to bring the pH to 8.5.
Has anyone had this issue, or is there a specific protocol that works better for making the clearing solution (like dissolving either boric acid or sds first?). Thanks!