After incubating in focus clear, the protocol says to mount in a chamber covered with a Wllco dish. Has anyone tried using simple coverslips?
We have Corning 1 1/2 cover slips that are about the same thickness of the glass bottoms of the Willco dish (~.17mm). May give them a try, but maybe the refractive properties are different.
If anyone has come up with a fun mounting solution that works, I'd be happy to hear it!
@Jalal : did you get a sample worthy of imaging? I would be very interested to know what kind of fluorophore you're about to image!