We're having issues with removing the sample from the gel.
The gel is extremely rubbery and as a result there is often a significant amount of gel left behind on the sample.
Is anyone else experiencing this or could shed some light onto the reason as to why this would be occurring?
So, the bis makes the hydrogel stronger so it can be easily handled and so that larger samples especially don't fall apart. This is less of a worry when you already have a small, fragile sample, although generally incorporating bis is always better for hydrogel integrity.
It seems like the consistency of the gel differs between groups using CLARITY. I was wondering what the consistency is supposed to be like? After polymerization, my gel is more like pudding/flan.
I just put the 50ml falcon in a 37C waterbath after I have replaced oxygen by nitrogen. Is the tube supposed to be on a shaker while polymerizes?