I'm clearing whole brains and spinal cords from adult mice, passively. It seems to be going well so far- it's been about a month and there is definitely clearing happening. My question has to do with whether it is possible to "over"clear tissue with CLARITY. In my samples, the clearing isn't necessarily even across the tissue; obviously with regard to depth the outer regions will clear faster than the middle, but there are also a few areas that seem to have minimal clearing in a spinal cord that is almost entirely clear elsewhere (possibly because I didn't remove the dura). Should I be worried about leaving those mostly-cleared samples at 37C for the time it takes for those scattered areas to become clear? (Does that risk any damage to the areas that have already cleared?) Thanks!