I seem to be having a problem with imaging at high magnification. I need to image at 60X to evaluate spine densities but even at 40X, the objective seems to need to be so close to the coverslip that there is pressure which causes the sample to float away in the mounting media. Has anyone else had this issue? I would appreciate any suggestions/advice. I am only trying to image through a 200um thick section so this should not be a problem at all yet here we are.