The Optogenetics and Neural Engineering (ONE) Core of the University of Colorado has designed a new ETC chamber made mostly from ‘off the shelf’ plumbing parts. Compared to other chamber designs, this design is easy to fabricate, is less prone to leaking, is easily expandable, better secures the tissue, deals well with bubbles, and is inexpensive (less than $65/chamber). The clearing solution flows in the same direction as the current, and is therefore called Linear ETC. This leads to efficient clearing (half mouse brain in two days). More information can be found in the .pdf posted to dropbox below (no account necessary).https://www.dropbox.com/s/0r5ojcit6kgeh9l/Linear CLARITY Electrophoretic Tissue Clearing Chamber.pdf?dl=0
Please acknowledge the ONE Core facility in your publications. An appropriate wording would be:
"Engineering support was provided by the Optogenetics and Neural Engineering Core at the University of Colorado Anschutz Medical Campus, funded in part by the National Institute for Neurological Disorders and Stroke of the National Institutes of Health under award number P30NS048154."