Cooked brain made me sad

cytokine

We have just tried running clearing using a peristaltic pump and had the same unfortunate outcome that others have noted - the tubing cracked o/n, leaked, brain cooked, mop recruited from corner. I saw that someone suggested Excelprene TPE tubing - has anyone tried this and found that it stands up to 600rpm (gives me around 500mL/min) without cracking? Are there alternatives (pump, tubing, method, etc).
thank you.

SuSam

Hi, maybe you can try the method of C.A.P.T.U.R.E. (CLARITY-based Activity and Projection Tracking upon Recombination, http://capture-clarity.org/) published in CELL this year. It’s easier than ETC and have pretty good reproducibility.

cytokine

Susam. Thank you.