Tissue Sectioning AFTER clearing

balsorjlbalsorjl Posts: 6
Has anyone tried sectioning tissue AFTER clearing? I have seen some groups that section tissue after acrylamide hydrogel incubation and polymerization, but I would like to section after tissue has cleared completely.

First, I am trying to avoid freezing cleared tissue. Second, when I mount my samples to a microslicer for sectioning, my adhesive is absorbed into the entire block of tissue.



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