ETC setup - circulating PUMP for clearing solution

richik

Hi,

I have recently started performing CLARITY on Xenopus frog embryos. I was wondering what kind of circulation pump I could use to circulate the clearing buffer at flow rate of 1- 2 L/min? I only have a peristaltic pump which can circulate at 500ml/hr max.

Any suggestion about the particular pump's name and manufacturer would be really helpful!

Jake

Please find info here: http://logosbio.com/x_clarity/x_clarity/features.php

Leo

Flash across my mind. Why the sampes are under the perfusion? If the CLARITY chamer size was much bigger then..