I've read the prior posts re these problems. The only difference is that we're mixing our own acrylamide (sigma a9099-100g) and bis (sigma m7279-100g) and making our own 16% pfa solution. The va044 is the wako one.
Following protocol...Degas and nitrogen flush. Tight cap. Incubated at 37...and nothing. Made second batch changed pfa ph to 7.45 after reading posts recommending this. Nothing.
Took first batch out overnight from freezer and left at room temp in conicals planning to discard. No polymers.
Left first and second batch in single conical at 38 water bath overnight. Nothing.
So I wonder what next? Should we stick with commercial solutions. Are folks mixing their own? What should we look out for? Any advice is immensely appreciated. Thank you.