Anyone tried second round of staining?

mhurry

The CLARITY wiki states that you can wash out the first antibodies by washing in clearing solution overnight at 60°C. Anyone had any luck with removing the first lot of antibodies and reprobing the tissue?

Irene

Hi @mhurry I'm working on human brain tissue, to remove the antibody from my stained sample i need 14 days not an incubation overnight. After that I can stain again the sample with a different antibody. (You can find some information in: http://www.nature.com/srep/2015/150507/srep09808/full/srep09808.html)