Anti-GFAP in CLARITY

jose_jpr

I'll do a pilot experiment with a sample of rat. Had thought to use Anti-GFAP, but I don't know the concentration to use it. Has anyone used this antibody?

Adam

1:100 is usually where I start with most antibodies, and this worked with GFAP for me (in mouse tissue).

mhurry

i used 1:100 in human tissue and it worked well

Irene

I used 1:50 in human tissue, you can find my protocol here: http://www.nature.com/srep/2015/150507/srep09808/full/srep09808.html

PostgradAU

1:100 is normally a good place to start - although this will depend on the purity of the antibody you are using?

This table provides a pretty good overview of the different starting dilutions based on antibody purity.

https://www.antibodies.com/resources/technical-faq#Concentration

Do any of you still use anti-serum?!