Whole-brain clearing in 8% SDS

BJ2014BJ2014 Posts: 8
edited March 2015 in Hydrogel Embedding
Has anyone tried clearing a whole mouse brain in 8% SDS after hydrogel embedding? I don't have the resources to do PARS clearing, so I am thinking of putting a perfused brain in hydrogel overnight @ 4degC, followed by 3-4hr incubation @ 37degC water bath. Any thoughts on the plausibility of this?


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