CLARITY forum
Home› General

Using Clarity for Liver tissue

VortexamVortexam Posts: 8
in General
Hi,I am also trying to make liver tissue transparent.I have few questions?
A. what type of tissue one can use in case of liver, freshly dissected or formalin-PFA stored tissue?
B. Has someone already tried using this technique to make liver tissue transparent? Any tips or problems faced?
C. Does the liver need to be sliced into sections of 200um thick or an entire liver lobe can be used?
· Share on FacebookShare on Twitter

Comments

  • marine_dubois01marine_dubois01 Posts: 8
    Hi,
    I don't know if you found an answer but i tried using CLARITY method on liver.
    After 2 months on passif clearing the liver wasn't clear. However, on liver lobe with ETC chamber it was work.

    I think to obtain a transparency may be cut the liver by lobe.

    · Share on FacebookShare on Twitter
  • VortexamVortexam Posts: 8
    Thank you for the advice. Is it somehow possible to email you and make a collaboration. I would like to get details about the ETC setup you have.
    Best wishes
    Amruta
    · Share on FacebookShare on Twitter
Sign In or Register to comment.