CLARITY and FISH

Technically, could the tissue went through CLARITY be used for FISH (Fluorescence in situ hybridization)? As far as I know the size of a regular DNA probe is smaller than that of a common antibody. Would the hydrogel fit for the other conditions required for FISH? What are the precautions would be? Thank you!

Comments

  • AdamAdam Posts: 31
    To resurrect this, has anyone tried FISH with CLARITY, other than the single figure panel in the original paper?
  • festevesfesteves Posts: 1
    Necro once more. Any word on this ?
  • If you are working with tissue sections, you will find an improvement in imaging performance by simply using a few drops of EasyIndex on the section, post staining. This will increase your signal to noise ratio also. EasyIndex rapidly clears sections less than 20um. A clearing protocol is not necessary for this range of tissue thickness.
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