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Electrophoretic Tissue Clearing (ETC)
Anti-GFAP in CLARITY
I'll do a pilot experiment with a sample of rat. Had thought to use Anti-GFAP, but I don't know the concentration to use it. Has anyone used this antibody?
1:100 is usually where I start with most antibodies, and this worked with GFAP for me (in mouse tissue).
i used 1:100 in human tissue and it worked well
I used 1:50 in human tissue, you can find my protocol here:
1:100 is normally a good place to start - although this will depend on the purity of the antibody you are using?
This table provides a pretty good overview of the different starting dilutions based on antibody purity.
Do any of you still use anti-serum?!