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Electrophoretic Tissue Clearing (ETC)
CLARITY + iDISCO
Is anyone doing iDISCO imaging with CLARITY or PACT samples?
iDISCO is a staining/clearance technique, do you mean using a lightsheet/SPIM system for imaging?
Yes! I'm sorry. Have you done it?
Yes, we have built the OpenSPIM system and currently use it for Clarity imaging.
Hi, I'm curious as to your experience with OpenSPIM and CLARITY. I'm currently building and OpenSPIM system and I'm planning on using it for CLARITY samples. What size of samples are you using? how are you putting the sample in front of the objective? Any information would help. Thank you for your time.