I don't know how the pancreas will be cleared with Clarity, but you should know that the tissue is not supposed to be completely transparent after the ETC. Your sample looks actually almost good enou… (View Post)
OK, so here is what I've got for the cortices : https://docs.google.com/file/d/0Byy2Hiz1XfrdYVZsVE1rS0xsRzQ/edit?usp=sharing You can see that 1 week of passive clearing is almost as good for transpar… (View Post)
To answer Laura, I just followed your recipe, and had very good results (except autofluorescence). The only change I made was to replace the sodium diatrizoate with meglumine diatrizoate, and had sli… (View Post)
The best suggestion I could give you : don't use a perfusion pump ! Those are too slow, and can't clear the chamber fast enough. You will have the temperature building up in the chamber as well, as y… (View Post)
@Fabio & @joelrosiene : Here you can see a summary of some tests I did : https://docs.google.com/file/d/0Byy2Hiz1XfrdRWcxTUtxa3pzQXM/edit?usp=sharing My current focusclear recipe is not all bad, … (View Post)