My guess would be that my samples would not tolerate this protocol very well. I would also be concerned about distortion of internal structures (which CLARITY already does to an extent) with that muc… (View Post)
Quick note on your 4th question - if you are interested in continuing with liver samples, you may want to image in 40% glycerol rather than 80%. Liver tends to have a lot of native background fluores… (View Post)