I'm using a Cole-Parmer EW-77910-30 peristaltic pump (~$900 new) set at about 150 ml/min to pump buffer from a 2L bottle in a 37C water bath into the chamber. The chamber then drains back into the bo… (View Post)
After I perfuse the mouse, I put the brain in a 20 mL tube that is completely filled with the PFA/acrylamide/bis-acrylamide-activator so there is little to no air in the tube. I store it at 4C for 3+… (View Post)
By the way, you can find cheaper platinum on Amazon. I bought some from SIgma and a supplier on Amazon. They were identical except the Amazon wire was 1/2 the price. (View Post)