Hi everyone, I just wanted to send a reminder that the Deisseroth lab offers training workshops on the CLARITY technique if you'd like a chance to learn and troubleshoot in-person. More information c… (View Post)
Clearing time with ETC can depend a lot on your specific ETC set up and settings (voltage, temperature, flow rate). 9 days is not unreasonable for good clearing. Longer incubation in SDS should not d… (View Post)
The hydrogel itself will probably have a similar refractive index as water. The refractive index of the tissue is determined by all the biomolecules attached inside the embedded tissue. (View Post)
I agree with @Fabio that lipid saturation is very unlikely to be your problem. You may just need to run your sample for a longer time... every set up is a little bit different, so you'll likely have … (View Post)