We don't yet know of a foolproof way to determine when clearing is finished, but when in doubt, I would leave the tissue in the ETC chamber an extra day or two. The brain will most likely not be comp… (View Post)
Keeping a flow of the clearing solution in the chamber throughout ETC is essential to preventing bubble accumulation in the brain sample. If you have no flow, the bubbles created during ETC may becom… (View Post)