Well got time to take a quick picture. Here is a cleared brain. The brain is floating in the solution, so the distance to the text is maybe to big. Still is a good clearing.
https://drive.google.com/… (View Post)
Leaving the bath open doesnt cahnge color (for me). You have evaporation and loss of buffer volume, which has an effect on ion concentration and SDS micelles.
The problems 1 & 2 & 3, I would… (View Post)
I can also add that I see already autofluorescence after hydrogel embedding.
I have a strong expression of eGFP in my brain. I can see it already in the brain
under a microscope without any clearing… (View Post)