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MariaSuessmilch

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MariaSuessmilch
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  • Hi. When I did the workshop we always mounted our sample in a way that they were immovable. So, we pushed the willco dish down so much that it touched the sample and held it in place. Here's more detail: We had a barrier of bluetac (putty) on a gla…
  • Kim wipes are absolutely essential in getting the hydrogel layer off of your sample. I remove as much of the gel as I can by hand and then use the Kim wipes, which grab onto the gel. You have to be careful but it works very well, at least on whole b…
  • Did blocking made a difference for you? I don't have this step in my original protocol... Also, can I ask which antibodies exactly you used? So which company it's from and what product code it has?
  • Hi, I'll walk you through what I did, which worked fine: Hydrogel solution: PREPARE ALL ON ICE and KEEP AT -20 degrees C. 4% acrylamide, 0.05% bis-acrylamide, 0.25% VA-044, 1x PBS, 4% PFA and distilled water. (PFA is crucial in this step as this …
  • Hey Clara, I am wondering whether you have tried this method yet and if so, did you have any luck with it?