I'm trying to use Clarity in my lab and I started using homemade PFA, I wanted to see if the polymerization was working before I use a sample, I put the hydrogel solution in a tube, added mineral oil on top and after 3 hours at 37°C you can see a gel, but with little bubbles on it. Has anyone saw this before?
what would it happen if I use a tissue sample?
Is a problem with the homemade PFA or because I'm not degassing?
Thank in advance for your help