ETC clearing end point

This is just a quick question I wanted to ask : how can we know when the ETC clearing is complete ? How the tissue (brain) should look at the end of this step? More specifically :

- In the first runs I did, I always end up with a yellowish tissue. Is this normal, or I am doing something wrong (Temp too high? Runs too long?). All the people I talked to have the same observation.

- Is the brain supposed to be transparent at this step? My brains are twice bigger after the ETC clearing, and are a light permissive, but definitely not "see through".

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