I'm in the process of getting everything together to start (attempt) the CLARITY protocol. I was wondering if anyone had tried using a wet transfer system designed for PAGE transfer for the ETC part of the protocol (A BioRad Mini-PROTEAN Tetra System). I planned on putting my tissue in plastic histology cassettes that are normally used for tissue processing and then trapping those in the electric field with the blot holder that slides into place. I would run tubes into and out of the chamber underneath the lid where there are already holes to prevent overfilling the chamber to allow for buffer circulation.
Is this a stupid idea for a reason I can't see at the moment?