There was some recent discussion on ResearchGate about whether the sample should be stationary or tumbling between the electrodes. Dr. Renier wrote that the authors told him that it should be tumbling. It seems intuitive, though, that the brain should be stationary to allow complete electrophoresis, rather than the lipids repeatedly moving back into the center.
What has been everyone's experience with this? Does anyone have an idea why tumbling might be recommended?