Imaging samples whose proteins could be denatured?

patriciapatricia Posts: 7
edited January 2014 in Sample Imaging
Hi everybody, I've cleared a kidney in the ETC chamber but during the weekend something happened with the electricity in the lab, and when I arrived the water pomp wasn't working, the power pac with error and the kidney had the renal fascia (the layer that covers the kidney) a little bit black, like burned. The thing is that the rest of the kidney seems to be well and transparent. Now I want to stain it, but I'm wondering if there is a possibility that the proteins of the kidney are denatured so I cannot get an specific staining, do you think it is possible? Thank you!

Comments

  • yetitiyetiti Posts: 7
    Denaturing itself shouldn't be a problem for antibody staining but the other damage caused by overheating could hurt staining.
  • Thank you yetiti.
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