I'd like to share some of my recent experience.
When I run my sample at 37C @ 30V, I noticed that some whitish layer starts forming on the tissue (only random spots). I was wondering if anyone had a similar experience and if this "precipitate" affects the further processing of the tissue (e.g. translucency after embedding).
I'm curious to hear about your thoughts.
PS.: The precipitate seems not to form if I run a flow rate of 15L/min. However, the buffer appears white (b/c of tiny bubbles). I'm not sure if this affects the clearing.