I was wondering if there are users on this forum that try to clear human postmortem brain tissue. In the paper it is stated that previous formalin-stored material (more than 6 yrs in formalin) was still suitable for CLARITY. I used Saponin in the hydrogel and tried passive clearing for a month on long-fixed (>1 yr) and short fixed (72hrs) material and there seems to be a large difference in clearing of the samples, where no clearing has been observed in 200 um long-fixed material compared to (beginning of) clearing of 3 mm chunck of short-fixed material.
Does anybody have similar experiences? Would it be advisable to switch to ETC for long-fixed material?