Unelectrophoresed, but clear...

We randomly checked on this hydrogel perfused/embedded right hemisphere of a rat cortex that was sitting in clearing solution in a 37 degree incubator for the last 5 weeks. We just had not gotten to doing ETC on it but it looks by far better than any of the brains that we have put through ETC, and with next to zero tissue damage. We had not changed the clearing solution in more than a month. We are planning on trying to duplicate ASAP and see if changing the solution more often has any positive effect. We are thinking this might be an option that, if producing mass amounts of tissue, may end up being faster considering that your only limitation on brains going at once is incubator space.



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