How to store the immunostained sample after imaging?


I am starting to try CLARITY and immunostaining on my 1-mm-thick mouse brain slices. I am wondering how do people store their immunostained samples after imaging in case they are needed for a second time imaging sometime later? I am concerned that if storing in PBS or clearing solution will let antibodies diffuse out over time and if that means the sample can only be imaged within a short period of time after immnostaining? Are there any tricks to prevent antibodies from leaking out of the sample?



  • yglassonyglasson Posts: 2
    I store my samples in PBS-azide0.1%. I can image them 1 month later without loosing of the signal!
  • xyangxyang Posts: 5
    Thanks a lot for your info!
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