Staining Neurons and counting them using CLARITY?

we are using the CLARITY technique to clear Rat brains and we would like to label astrocytes and neurons in the cerebral cortex and to be able to count them. We tried to stain it using NeuN and GFAP, but it appears that it didn’t stain when we did imaging. Do you have any recommendations/thoughts on why it did not work, or any specific steps we should follow?
we followed steps on
http://wiki.claritytechniques.org/index.php/Immunostaining

Comments

  • aysegulaysegul Posts: 33
    Hi maryam_z, how many days did you incubate your section in primer antibody and how much antibody dilution did you use?
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