For anyone who has gotten to imaging yet, have you noticed a lot of autofluorescence in the green and red wavelength ranges? I am working with heart tissue, which I know is notorious for autofluorescence, but sounds like this may be true for other tissues as well. To try to get around this, I am testing blue and far-red dyes, but I would like to know other thoughts or suggestions.
Also, according to the SeeDB article posted about by Bertrand_Vernay on the FocusClear discussion (http://forum.claritytechniques.org/discussion/1/focusclear#latest
), this autofluorescence issue could be due to the Maillard reaction, which means it could potentially be prevented... however I don't think the chemicals used to do this would be practical to add to the ETC buffer during the clearing process (2-ME or 1-thiogylcerol). The Maillard reaction is reversible though.