SDS and acrylamide concentrations

Has anyone done ETC clearing with more than 4% SDS such as 8% or 20% SDS? Did this result in faster clearing time and was it safe for the tissue?

Also, has anyone tried to use 8% SDS and 1% acrylamide (in the HM solution) successfully and what was the maximum time needed to clear 1/2 of a brain?
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Comments

  • Perhaps, 1mm clearing/day, from wall of tissue. i get a clearing with 8 and 10% SDS solution.
    good luck.
  • Thanks. Do you get faster clearing when using 10% SDS instead or 8% or 4% SDS?
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